lef1 (Proteintech)
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Proteintech
lef1
Lef1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+lef1/LEF1+Antibody/pmc13039260-65-34-36
Average 94 stars, based on 75 article reviews
Lef1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+lef1/LEF1+Antibody/pmc13039260-65-34-36
Average 94 stars, based on 75 article reviews
lef1 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1"
Article Title: β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1
Journal: Cell Death & Disease
doi: 10.1038/s41419-026-08457-y
Figure Legend Snippet: A Correlation between OXCT1 and CTNNB1 (encoding β-catenin) RNA expression in HCC samples from the TCGA dataset. B Western blot analyzed the protein levels of β-catenin and OXCT1 in HCCLM3 and Huh7 cells. C , D The mRNA and protein levels of β-catenin and OXCT1 were analyzed by Western blot or RT-qPCR in HCCLM3-β-catenin S33Y (β-catenin) or control (CON) cells. E , F The mRNA and protein levels of β-catenin and OXCT1 were analyzed by Western blot or RT-qPCR in Huh7 cells transfected with shRNA of CTNNB1 (shCTNNB) or no-targeting control (shCON). G Correlation between OXCT1 and LEF1 mRNA expression in HCC samples from the TCGA database. H Western blot analyzed protein levels of OXCT1 and LEF1 in HCCLM3 cells transfected with LEF1 plasmid or empty vector (EV). ( I ) Western blot analyzed protein levels of OXCT1 in HCCLM3 cells transfected with LEF1 shRNA (shLEF1) or β-catenin S33Y vector. J Co-IP assay demonstrating the interaction between β-catenin and LEF1 in Huh7 cells. K ChIP assay was performed with LEF1 antibody. IgG was used as the negative control. Chip-PCR was conducted at the promoter regions of OXCT1 with Huh7 cells. L Dual-luciferase reporter assays in HEK293 cells. Cells were co-transfected with a wild-type (PGL3-OXCT1-WT) or mutant (PGL3-OXCT1-MT) OXCT1 promoter reporter, along with expression plasmids for β-catenin, LEF1, TCF4+β-catenin, or LEF1 shRNA, as indicated. Luciferase activity was measured 48 h post-transfection. M Schematic of the OXCT1 promoter region. The consensus LEF1-binding site (5′-ACTTTGAACT-3′) is indicated. Data are presented as mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ns, not significant.
Techniques Used: RNA Expression, Western Blot, Quantitative RT-PCR, Control, Transfection, shRNA, Expressing, Plasmid Preparation, Co-Immunoprecipitation Assay, Negative Control, Luciferase, Mutagenesis, Activity Assay, Binding Assay
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